MATLAB is a comprehensive software package for scientific computing. We use MATLAB to analyze and fit data, to perform simulations, and to analyze images.
Kalaimoscope is software dedicated to tracking moving objects in microscope images. These objects can be single molecules, whole nuclei, whole cells, and anything in between. Each object is represented as by summed Lorentzian functions and tracked over time. The characteristics of the objects and trajectories can be used as readouts for underlying biochemical and biophysical mechanisms.
OriginPro is the world's best curve-fitting and statistics analysis software.
Mathematica allows us to solve complicated symbolic math problems that are tedious by hand.
Metamorph is the image acquisition software that drives our microscopes and our first line of analysis for looking at our images, measuring pixel intensities, etc.
Our lab uses two Zeiss TIRF microscopes. These microscopes are the core tool of the lab. We like Zeiss for their high quality objective lenses and the overall reliability of their equipment.
Single-molecule biophysics would not be possible without electron-multiplying charged couple device (EMCCD) cameras. We have chosen Andor EMCCD cameras for their best-in-class performance and low noise levels.
When the data is ready to publish, we use Adobe Illustrator to prepare the highest-quality scientific figures.