— Publications

Selected Publications

The structure and dynamics of C. elegans tubulin reveals the mechanistic basis of microtubule growth.

Chaaban S, Jariwala S, Hsu CT, Redemann S, Kollman JM, Muller-Reichert T, Sept D, Bui KH, Brouhard GJ. (2018) Developmental Cell. 23(1): 181-92.
PMID: 30245157
Microtubule dynamics: an interplay of biochemistry and mechanics.

Brouhard GJ, Rice LM. (2018) Nature Reviews Molecular Cell Biology. 19(7): 451-163.
PMID: 29674711
A microtubule bestiary: structural diversity in tubulin polymers.

Chaaban S, Brouhard GJ. (2017) Molecular Biology of the Cell. 28(22): 2924-2931.
PMID: 29084910
The synthetic diazonamide DZ-2384 has distinct effects on microtubule curvature and dynamics without neurotoxicity.

Wieczorek M, Tcherkezian J, Bernier C, Prota AE, Chaaban S, ..., Steinmetz MO, Shore GC, Brouhard GJ, Roulston A. (2016) Science Translational Medicine. 8(365):365ra159.
PMID: 27856798
Microtubule-associated proteins control the kinetics of microtubule nucleation.

Wieczorek M, Bechstedt S, Chaaban S, Brouhard GJ. (2015) Nature Cell Biology. 17(7): 907-16.
PMID: 26098575
Doublecortin recognizes the longitudinal curvature of the microtubule end and lattice.

Bechstedt S, Lu K, Brouhard GJ. (2014) Current Biology. 24(20): 2366-75.
PMID: 25283777
The contribution of αβ-tubulin curvature to microtubule dynamics.

Brouhard GJ, Rice LM. (2014) Journal of Cell Biology 207(3): 323-34.
PMID: 25385183
Doublecortin recognizes the 13-protofilament microtubule cooperatively and tracks microtubule ends.

Bechstedt S, Brouhard GJ. (2012) Developmental Cell. 23(1): 181-92.
PMID: 22727374

All Publications

The structure and dynamics of C. elegans tubulin reveals the mechanistic basis of microtubule growth.

Chaaban S, Jariwala S, Hsu CT, Redemann S, Kollman JM, Muller-Reichert T, Sept D, Bui KH, Brouhard GJ. (2018) Developmental Cell. 23(1): 181-92.
PMID: 30245157
Microtubule dynamics: an interplay of biochemistry and mechanics.

Brouhard GJ, Rice LM. (2018) Nature Reviews Molecular Cell Biology. 19(7): 451-163.
PMID: 29674711

A microtubule bestiary: structural diversity in tubulin polymers.

Chaaban S, Brouhard GJ. (2017) Molecular Biology of the Cell. 28(22): 2924-2931.
PMID: 29084910

The synthetic diazonamide DZ-2384 has distinct effects on microtubule curvature and dynamics without neurotoxicity.

Wieczorek M, Tcherkezian J, Bernier C, Prota AE, Chaaban S, ..., Steinmetz MO, Shore GC, Brouhard GJ, Roulston A. (2016) Science Translational Medicine. 8(365):365ra159.
PMID: 27856798
GTSE1 tunes microtubule stability for chromosome alignment and segregation by inhibiting the microtubule depolymerase MCAK.

Bendre S, Rondelet A, Hall C, Schmidt N, Lin YC, Brouhard GJ, Bird AW. (2016) Journal of Cell Biology. 215(5):631-647.
PMID: 27881713

Microtubule-associated proteins control the kinetics of microtubule nucleation.

Wieczorek M, Bechstedt S, Chaaban S, Brouhard GJ. (2015) Nature Cell Biology. 17(7): 907-16.
PMID: 26098575

The contribution of αβ-tubulin curvature to microtubule dynamics.

Brouhard GJ, Rice LM. (2014) Journal of Cell Biology 207(3): 323-34.
PMID: 25385183
Doublecortin recognizes the longitudinal curvature of the microtubule end and lattice.

Bechstedt S, Lu K, Brouhard GJ. (2014) Current Biology. 24(20): 2366-75.
PMID: 25283777
Collapsin Response Mediator Protein 4 Regulates Growth Cone Dynamics through the Actin and Microtubule Cytoskeleton.

Khazaei MR, Girouard MP, Alchini R, Ong Tone S, Shimada T, Bechstedt S, Cowan M, Guillet D, Wiseman PW, Brouhard G, Cloutier JF, Fournier AE. Journal of Biological Chemistry. 2014 Oct 24;289(43):30133-43.
PMID: 25225289
Microtubules accelerate the kinase activity of Aurora-B by a reduction in dimensionality.

Noujaim M, Bechstedt S, Wieczorek M, Brouhard GJ. PLoS One. 2014 Feb 3;9(2):e86786
PMID: 24498282

Macromolecular crowding pushes catalyzed microtubule growth to near the theoretical limit.

Wieczorek M, Chaaban S, Brouhard GJ. (2013) Cellular and Molecular Bioengineering 6: 383-392.
DOI: 10.1007/s12195-013-0292-9
Motors and MAPs collaborate to size up microtubules.

Bechstedt S, Brouhard GJ. Developmental Cell 2013; ed: pp.
PMID: 23906062
Fluorescence-based assays for microtubule structure.

Bechstedt S, Brouhard GJ. Methods in Cell Biology 2013; ed: pp.
PMID: 23973082
Measuring ligand-receptor binding kinetics and dynamics using k-space image correlation spectroscopy.

Brandão HB, Sangji H, Pandžić E, Bechstedt S, Brouhard GJ, Wiseman PW. Methods 2013; ed: pp.
PMID: 23938869
Polymicrogyria with dysmorphic basal ganglia? Think tubulin!

Amrom D, Tanyalçin I, Verhelst H, Deconinck N, Brouhard G, Décarie JC, Vanderhasselt T, Das S, Hamdan F, Lissens W, Michaud J, Jansen A. Clinical Genetics 2013; ed: pp.
PMID: 23495813

Doublecortin recognizes the 13-protofilament microtubule cooperatively and tracks microtubule ends.

Bechstedt S, Brouhard GJ. Developmental Cell 2012; ed: pp.
PMID: 22727374
Microtubules: sizing up the GTP cap.

Brouhard G, Sept D. Current Biology 2012; ed: pp.
PMID: 23017994

Variations on the single-molecule assay for microtubule-associated proteins and kinesins.

Bechstedt S, Wieczorek M, Noujaim M, Brouhard GJ. Methods Mol Biol. 2011;777:167-76.
PMID: 21773928
XMAP215 polymerase activity is built by combining multiple tubulin-binding TOG domains and a basic lattice-binding region.

Widlund PO, Stear JH, Pozniakovsky A, Zanic M, Reber S, Brouhard GJ, Hyman AA, Howard J. Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):2741-6.
PMID: 21282620

Quality control in single-molecule studies of kinesins and microtubule-associated proteins.

Brouhard GJ.Methods Cell Biol. 2010;97:497-506.
PMID: 20719287
CLASP promotes microtubule rescue by recruiting tubulin dimers to the microtubule.

Al-Bassam J, Kim H, Brouhard G, van Oijen A, Harrison SC, Chang F. Dev Cell. 2010 Aug 17;19(2):245-58.
PMID: 20708587
Motor proteins: kinesins influence each other through load.

Brouhard GJ. Curr Biol. 2010 May 25;20(10):R448-50.
PMID: 20504755

Postdoctoral Publications

XMAP215 is a processive microtubule polymerase.

Brouhard GJ, Stear JH, Noetzel TL, Al-Bassam J, Kinoshita K, Harrison SC, Howard J, Hyman AA. Cell. 2008 Jan 11;132(1):79-88.
PMID: 18191222
The depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends.

Helenius J, Brouhard G, Kalaidzidis Y, Diez S, Howard J. Nature. 2006 May 4;441(7089):115-9.
PMID: 16672973
Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy.

Gell C, Bormuth V, Brouhard GJ, Cohen DN, Diez S, Friel CT, Helenius J, Nitzsche B, Petzold H, Ribbe J, Schäffer E, Stear JH, Trushko A, Varga V, Widlund PO, Zanic M, Howard J. Methods Cell Biol. 2010;95:221-45. Review.
PMID: 20466138
Dynamics of zebrafish somitogenesis.

Schröter C, Herrgen L, Cardona A, Brouhard GJ, Feldman B, Oates AC. Dev Dyn. 2008 Mar;237(3):545-53.
PMID: 18265021

Ph.D. Publications

Microtubule movements on the arms of mitotic chromosomes: polar ejection forces quantified in vitro.

Brouhard GJ, Hunt AJ. Proc Natl Acad Sci U S A. 2005 Sep 27;102(39):13903-8.
PMID: 16174726
Advanced optical tweezers for the study of cellular and molecular biomechanics.

Brouhard GJ, Schek HT 3rd, Hunt AJ. IEEE Trans Biomed Eng. 2003 Jan;50(1):121-5.
PMID: 12617534

Selected Publications

The structure and dynamics of C. elegans tubulin reveals the mechanistic basis of microtubule growth.

Microtubule dynamics: an interplay of biochemistry and mechanics.

A microtubule bestiary: structural diversity in tubulin polymers.

The synthetic diazonamide DZ-2384 has distinct effects on microtubule curvature and dynamics without neurotoxicity.

Microtubule-associated proteins control the kinetics of microtubule nucleation.

Doublecortin recognizes the longitudinal curvature of the microtubule end and lattice.

The contribution of αβ-tubulin curvature to microtubule dynamics.

Doublecortin recognizes the 13-protofilament microtubule cooperatively and tracks microtubule ends.

All Publications

The structure and dynamics of C. elegans tubulin reveals the mechanistic basis of microtubule growth.

Microtubule dynamics: an interplay of biochemistry and mechanics.

A microtubule bestiary: structural diversity in tubulin polymers.

The synthetic diazonamide DZ-2384 has distinct effects on microtubule curvature and dynamics without neurotoxicity.

GTSE1 tunes microtubule stability for chromosome alignment and segregation by inhibiting the microtubule depolymerase MCAK.

Microtubule-associated proteins control the kinetics of microtubule nucleation.

The contribution of αβ-tubulin curvature to microtubule dynamics.

Doublecortin recognizes the longitudinal curvature of the microtubule end and lattice.

Collapsin Response Mediator Protein 4 Regulates Growth Cone Dynamics through the Actin and Microtubule Cytoskeleton.

Microtubules accelerate the kinase activity of Aurora-B by a reduction in dimensionality.

Macromolecular crowding pushes catalyzed microtubule growth to near the theoretical limit.

Motors and MAPs collaborate to size up microtubules.

Fluorescence-based assays for microtubule structure.

Measuring ligand-receptor binding kinetics and dynamics using k-space image correlation spectroscopy.

Polymicrogyria with dysmorphic basal ganglia? Think tubulin!

Doublecortin recognizes the 13-protofilament microtubule cooperatively and tracks microtubule ends.

Microtubules: sizing up the GTP cap.

Variations on the single-molecule assay for microtubule-associated proteins and kinesins.

XMAP215 polymerase activity is built by combining multiple tubulin-binding TOG domains and a basic lattice-binding region.

Quality control in single-molecule studies of kinesins and microtubule-associated proteins.

CLASP promotes microtubule rescue by recruiting tubulin dimers to the microtubule.

Motor proteins: kinesins influence each other through load.

Postdoctoral Publications

XMAP215 is a processive microtubule polymerase.

The depolymerizing kinesin MCAK uses lattice diffusion to rapidly target microtubule ends.

Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy.

Dynamics of zebrafish somitogenesis.

Ph.D. Publications

Microtubule movements on the arms of mitotic chromosomes: polar ejection forces quantified in vitro.

Advanced optical tweezers for the study of cellular and molecular biomechanics.